U predavaonici Instituta u utorak 21.11.2017. u 13:30 sati održat će se kolokvij prof. Vladimir M. Mirsky iz Nanobiotechnology – Institute of Nanobiotechnology, Brandenburgsische Technische Universität Cottbus – Senftenberg, Njemačka.
Naslov predavanja je Ultrasensitive detection, quantification and identification of nanoparticles
Permanent growth in the production of engineered nanoparticles increases risk of human contacts with these potentially hazardous materials. It makes important a development of sensitive analytical methods for quantification and identification of nanoparticles in liquid and gaseous media. For this purpose, a method based on the detection and analysis of minute signals of surface plasmon resonance (SPR) images due to adsorption of single nanoparticles was developed. This new technology allows one a real-time detection of interaction of single nanoparticles with sensor surface. Adsorption of each nanoparticle leads to characteristic diffraction image whose shape and intensity depends on the size and chemical composition of nanoparticle. A number of the nanoparticle–surface binding events per time and surface area characterizes volume concentration of nanoparticles. A large monitored surface area of sensor surface allows one to detect many hundreds events in each frame, this leads to a very high dynamic range of counting and to a correspondingly high dynamic range in the concentration scale. Depending on the type of nanoparticles and experimental conditions, the detection limit for aqueous samples can be below 1000 nanoparticles per microliter. Statistical analysis of images of nanoparticles provides information on heterogeneity of nanoparticles and can be used as fingerprints for identification of different types of nanomaterials. Chemical functionalization of the sensor surface as well as changes of pH or ionic strength are additional factors influencing the behavior of nanoparticles and allowing one their identification. Independent information on chemical composition and size of nanoparticles can be obtained from SPR visualization of their electrochemical dissolving or modification during potential sweep. The method was also applied for ultrasensitive detection and analysis of nanoparticles in very complex media, such as tap water, sunscreen, juice or wine. Beside analytical applications, the new approach provides valuable scientific information on adsorptive properties of nanoparticles which can be used to predict their toxicity or to study interaction of nanoparticles with surfaces.
Kratki životopis predavača
Prof. V. Mirsky is full professor for Nanobiotechnology since October 2008 and came from University of Regensburg where he was Professor in the Institute of Analytical Chemistry, Chemo- and Biosensors at the University of Regensburg. The scientific activity of Prof. Mirsky (www.b-tu.mirsky.de) is focused on different aspects of surface science, material science, and measurement technology required for the development of biological and chemical sensors: electrically, thermodynamically, or kinetically controlled formation of self-assembled monolayers, improvement of different techniques for immobilization of biomolecules, formation of receptor arrays, optimization of microcontact printing, new approaches for formation of nanostructured monolayers, application of metallic nanoparticles in biosensing, characterization of chemosensitive properties of electrically conducting polymers and their analytical applications, implementation of high-throughput techniques for development of chemosensitive materials, inorganic conductometric gas-sensors, molecularly-imprinted polymerization, smart labels, development of different biosensors based on impedometric, and plasmonic transducing. The results of these investigationas are presented in about 140 peer-reviewed publications, three (co)edited books and led to numerous patent applications. He has large experience of cooperation with industry (including such companies as Bosch, Siemens, Solvay and numerous SMEs). He has prepared and managed a number of large multi-disciplinary projects for industry and for national and international funding including EU FP 7 project NANODETECTOR (10 participants from 7 countries, coordinator), Volkswagen Foundation (3 countries, 5 participants, coordinator), NATO (3 countries, 3 participants, coordinator), EU-FP4 and FP6 projects (principal investigator from the team of his university), international BMBF project COMPONET (coordinator), national BMBF-Project KOMBISENS and a number of DFG-projects.