Archives: Projekti

IMI projekti

Toxicity Assessment in 2D vs 3D cultures of Zebrafish liver cells (ZebTox3D)

Ministry of Science, Education and the Youth, Croatian-Slovenian Bilateral Cooperation, 2025-2026

Project Leader:

Leader in Slovenia:

  • Matjaž Novak, National Institute of Biology (NIB), Ljubljana, Slovenia

Participants (IMROH):

Participants (NIB):

  • Bojana Žegura
  • Alja Štern
  • Katja Kološa
  • Sonja Žabkar
  • Iza Rozman

Summary
The zebrafish (Danio rerio) is one of the most important ecotoxicological models for studying the effects of pollutants. In accordance with the 3R principles (Reduce, Refine, Replace), in vitro models utilizing fish cell lines have been developed for xenobiotic toxicity assessment. Zebrafish liver epithelial cells (ZFL) possess the ability to metabolize various compounds and are widely used in toxicological research. However, data obtained from two-dimensional (2D) cultures have shown limitations in predicting in vivo responses, making 3D cultures a more relevant model due to their enhanced cellular differentiation, morphology, and gene expression. While 3D cultures are frequently used in mammalian toxicology studies, their application in ecotoxicology remains scarce. This project aims to establish ZFL 3D cultures as a model for toxicity assessment and to compare the responses of 2D and 3D systems to various pollutants, including bisphenols, nanoparticles, pharmaceutical residues, and polycyclic aromatic hydrocarbons. Cytotoxic and genotoxic effects will be evaluated through assays assessing cell viability, proliferation, migration, induction of cell death, and DNA damage. Comparing results between 2D and 3D models will enable the identification of the most suitable system for predicting in vivo responses. The project builds on a long-standing collaboration between Slovenian and Croatian research groups, leveraging their complementary expertise to advance in vitro methodologies in ecotoxicology. The expected outcomes of this research will contribute to new scientific insights, foster industry collaborations, and facilitate the education of young researchers. The findings will serve as a foundation for future international project applications, including those within the Horizon EU program.

 

EvaMobs – Evolvable and rapidly adaptable monobodies: a broad-spectrum antiviral platform

Consortium leader: Claudio M. Soares (ITQB Nova, Lisbon, Portugal)

Work package (WP3): Maja Katalinić

Participants (IMI): Antonio Zandona, Suzana Žunec

Programme: HORIZON

Call: HORIZON-HLTH-2023-DISEASE-03-04

Duration: 1. 1. 2024. – 31. 12. 2028.

Abstract
With progress in globalization, expansion of human populations into natural habitats, and aggravation of climate change comes an increased risk of viral outbreaks. As demonstrated by the COVID-19 pandemic, not being prepared for such events has devastating consequences on public health, society and the economy. EvaMobs will improve preparedness of the European Union (EU) for the next viral outbreak(s) of pandemic potential by developing a platform for the discovery, development, production and validation of evolvable and rapidly adaptable antivirals. These innovative medicines will be based on small human-derived proteins called monobodies (Mobs). As Mobs can be engineered to have high binding affinity for virtually any viral protein, this platform can be easily adapted to a broad range of viruses, including newly emerging viruses and viral variants. To demonstrate the capacity of this platform it will first be applied to four pathogenic viruses with epidemic and/or pandemic potential: Influenza A, SARS-CoV-2, respiratory syncytial virus, and Zika virus. Deep-learning and computational design tools will allow generation of tailor-made Mobs with cryo-EM elucidating the molecular details of their binding interaction. Simple bacterial expression of Mobs, the development of a semi-automated high-throughput screening platform for evaluation of the Mobs’ stability and target affinity and streamlined in vitro and in vivo preclinical validation, will allow rapid development and selection of stable and potently neutralizing candidates. The Mob with the best preclinical indicators will then be tested in a phase I clinical trial after implementing a stable formulation and GMP production. The optimized platform can then be adapted to other viruses. Therefore, EvaMobs provides an innovative, robust and flexible platform for antiviral biologics development as well as a diverse portfolio of validated drugs, strengthening the EU’s pandemic preparedness.

Link: https://evamobs.eu/

 

Effects of exposure to plasticizers and microplastics on men’s reproductive health

Croatian Science Foundation: Research project IP-2024-05-8815

Duration: 36 months, 16 Dec 2024 – 15 Dec 2027

Abstract
Along with the well-known phthalate esters (PAE), organophosphate esters (OPE) are a less researched class of plasticizers. Due to their properties, they are frequently added to consumer products, which is the main reason of the compounds’ widespread migration in the environment. The detection of PAEs and OPEs in the urine of the general public (both children and adults) has drawn the attention of scientists and regulatory agencies responsible for protection of human health and environment. Furthermore, the global rise in the use of disposable plastic items has led to an escalation in human contact with microplastics (MP). One of the main objectives of the PlastRepRisk project is to establish an analytical platform for analysing plasticizers (OPE, PAE) and MP particles in complex matrices such as human blood serum and urine. Main goal is to obtain preliminary data on the levels of exposure of the general population in Croatia to OPE and PAE compounds and MP particles. Although their health effects have not yet been fully elucidated, certain OPE and PAE compounds, as well as MPs, are suspected to act as endocrine disruptors and/or have detrimental effects on reproductive health. This study will also evaluate the risks to male reproductive health by comparing men diagnosed with infertility to healthy (fertile) subjects. Correlations between the concentrations of specific OPE, PAE, and MPs in blood/urine and infertility defined as a low count and/or poor quality of sperm and disruption in sex hormone levels will be assessed in order to investigate the hypothesis that exposure to plasticizers/MP may adversely affect reproductive function. The research will provide key insights into the possible impact of OPE, PAE, and MPs exposure on reproductive health disorders under actual exposure levels, in addition to obtaining first data on the levels of exposure of the Croatian population (men of reproductive age) to these compounds/particles.

Informing beekeepers about quality propolis (InfoBeePro)

Duration: 10 May 2024–31 Jan 2025

Total funding: 2,500.00 EUR

Donation from Hrvatska elektroprivreda d.d.: 1,400.00 EUR

Summary: Propolis has a high content of phenolic compounds that significantly contribute to its antimicrobial, anti-inflammatory, antioxidant, and immunomodulatory effects. Therefore, the quality of propolis significantly depends on its phenolic composition. In addition to plant resins, bees can also collect various substances available in the environment, such as toxic elements (e.g. lead, cadmium, arsenic, mercury, etc.) that can be found in propolis. The aim of this project is to determine the level of toxic elements and bioactive components in propolis samples that will be collected in agreement with beekeepers from the Istrian and Primorje-Gorski Kotar counties. The main beneficiaries of the project will be beekeepers who collect propolis, and the results will be implemented through education on measures to reduce the negative impact of the environment on propolis and procedures for preparing propolis extracts with the highest content of bioactive components. Also, propolis consumers will be informed about the quality of Croatian propolis after determining the level of toxic and essential elements, phenolic compounds and antioxidant activity of the collected propolis samples. Additional propolis analyses are planned at locations where an increased proportion of toxic elements is determined, after implementing corrective measures aimed at reducing anthropogenic impacts (as part of field surveys). All research results will be based on modern scientific methods and will make a significant contribution to public health with the possibility of implementing the results in the Croatian economy for the general and national good, which is in line with the vision and mission of the Institute.

 

Analyses of interactions between organophosphorus compounds and esterases and other targets for therapy in poisoning (OPEsterOX)

Croatian Science Foundation: Research project IP-2022-10-6885

Team members: Goran Šinko, Tena Čadež, Dora Kolić, Višnja Stepanić, Vesna Gabelica Marković, Zoran Radić

Duration: 48 months, 2023-2027

Abstract

Exposure to organophosphates (OP) from both pesticides and nerve agents leads to the covalent inhibition of the pivotal enzyme acetylcholinesterase (AChE) that leads to accumulation of neurotransmitter acetylcholine, which induces cholinergic crisis, that is, overstimulation of muscarinic and nicotinic membrane receptors in the central and peripheral nervous system. In severe cases, subsequent desensitisation of the receptors results in hypoxia, vasodepression, and respiratory arrest, followed by death. Standard therapy of OP poisoning that generally involves administration of anticholinergic atropine and an oxime reactivator of phosphylated AChE has limitations especially in the case of phosphoroamidates due to the structural-related mechanism of reactivation. An alternative therapy is the related enzyme butyrylcholinesterase (BChE) which binds OP compounds and as such acts as stoichiometric bioscavenger of OP. Therefore, this project utilizes known (e.g. tabun, methamidophos and fenamiphos), and new compounds (A-agents) to gain a better understanding of the mechanistic basis of cholinesterase family interactions and their limitations to find new effective leads for treatment. The biochemical mechanism of enzyme interactions will be comprehensively studied on a molecular level with in silico, in vitro, and ex vivo methods to define binding affinities, kinetic constants of inhibition and efficiency of reactivation of both enzymes. The possible mechanisms of toxicity of selected OPs will be studied at cell-culture level, and on mice exposed to OP. The neuroinflammatory effect of OP as w ell as the neuroprotective effectiveness of oxime therapy will be examined on the mouse brain by monitoring specific markers of astrogliosis, microgliosis and neuron viability. These comprehensive analyses will undoubtedly contribute to the knowledge of phosphoroamidate interactions and improved therapeutics in OP poisoning.

Publications
  1. Kolić, Dora; Kovarik, Zrinka. NMDA receptors: structure, function and role in organophosphorus compound poisoning // Biofactors, in press (2024), doi: 10.1002/biof.2048

Environment pollution and human health: physico-chemical analysis, toxicity, and machine learning models

Acronym: EnvironPollutHealth

Duration: 1 Jan 2024–31 Dec 2027

Funded by the European Union – Next Generation EU
– Program Contract of 8 Dec 2023, Class: 643-02/23-01/00016, Reg. no. 533-03-23-0006
Funding: 460,652.00 EUR

Holder: Institute for Medical Research and Occupational Health, Division of Environmental Hygiene

Partners:

  • University of Belgrade, Faculty of Chemistry, Department of Applied Chemistry (Belgrade, Serbia)
  • University of Belgrade, Institute of Physics, Environmental Physics Laboratory (Belgrade, Serbia)
  • University of Zadar, Department of Health Studies (Zadar, Croatia)
  • University of Zadar, Department of Ecology, Agronomy and Aquaculture (Zadar, Croatia)
  • University of Zagreb, Faculty of Agriculture, Department of General Agronomy (Zagreb, Croatia)
  • University of Zagreb, Faculty of Agriculture, Department of Soil Amelioration (Zagreb, Croatia)
  • University of Zagreb, Faculty of Mining Geology and Petroleum Engineering, Department of Mineralogy, Petrology and Mineral Resources (Zagreb, Croatia)
  • University of Zagreb, Faculty of Forestry and Wood Technology, Institute of Processes Engineering (Zagreb, Croatia)
  • Institute for Anthropological Research, Laboratory for Chemical Analytics (Zagreb, Croatia)
  • AQUATIKA – Karlovac Freshwater Aquarium (Karlovac, Croatia)

More info about the project

Interactions between Human and Environmental Health: Determinants of Health Preservation – HumEnHealth

More info about the project available at: https://projekti.imi.hr/humenhealth/en/

This project, in line with the One Health approach of the World Health Organization, aims to integrate research for a better understanding of the health status of specific environmental and animal systems. The goal is to understand their impact on human health, explore ways to detect health disorders in humans and the environment early, and identify methods for prevention. The research will encompass potential interactions among genetic, nutritional, and psychosocial factors contributing to health disorders caused by pollutants in the living and working environment. Additionally, the project will investigate possibilities to enhance the methodology of epidemiological studies and improve statistical data processing methods.

The project will focus on:
1. Chemical pollutants (metals, pesticides, biocides, skin allergens, and irritants).
2. Physical pollutants (noise).
3. Psychosocial factors (personal and organizational stressors, broader social context stressors).

The environmental burden will be analyzed in aquatic organisms, water, sediment, plants, and animals in terrestrial ecosystems and/or food. Biochemical mechanisms of nutrient and pollutant action will be explored using in silico methods. Human organism load will be studied through biomonitoring methods, investigating health disorders such as skin inflammation, obesity, osteoporosis, neurobehavioral disorders, developmental disorders, plant and animal poisoning, vitamin and mineral deficiencies, and hearing damage caused by noise, using clinical and psychological methods.

The main project goal is to investigate the health status of selected environmental and animal systems, their impact on human health, early detection methods for human and environmental health disorders, and ways to improve prevention.

Specific project objectives include:
1. Examining the distribution and accumulation of metal(oid)s in target organs of tissues in animal indicator species.
2. Assessing nutrient intake and exposure to major food and water pollutants, evaluating potential health risks and characterizing plant and fungal poisonings in the population.
3. Assessing metal(loid) exposure (Cd, Pb, Hg, As) via dietary and lifestyle habits in the general and vulnerable population using human biomonitoring; evaluating the association between toxic and essential elements and genetic polymorphisms, including epigenetic and metabolomic effects; and developing the new analytical methods for element determination in biological samples.
4. Investigation of the effects of genetic factors and environmental chemicals on the development of occupational skin diseases; estimating the skin barrier function parameters as early biomarkers of skin diseases; assessing the occupational health and safety status in high-risk populations; and conducting an educational intervention to improve the prevention of occupational skin diseases.
5. Studying recreational headphone use in young adults, measuring noise exposure and its potential association with hearing damage.
6. Identifying enzymatic reactions of tyrosinase at the molecular level for understanding the development and treatment of Parkinson’s disease.
7. Assessing sleep quality and daily functioning in different age groups in relation to chemical and psychosocial factors.
8. Examining and proposing statistical methods for analyzing data from various sources in the assessment of chemical exposure and harmful effects.

The research will be conducted through seven work packages, each focusing on specific aspects of the project:

Work Package 1. Environmental Health (leader Zorana Kljaković-Gašpić) Considering a wide range of factors and their complex interactions, this work package will investigate the levels of metal(oid)s (both essential and potentially toxic) in abiotic components of the environment (soil, water, and sediment) and bioindicator organisms at different trophic levels of aquatic and terrestrial food webs, including some rarely covered by monitoring programs (rare and/or protected species). The pathways of metal(oid) transfer and accumulation between different ecosystem components will be explored, depending on biological and ecological influencing factors. The connection between the content of elements in target tissues of accumulation, indicators of oxidative stress (effect biomarkers), protein indicators of exposure (metallothionein, MT), and hormones in blood and hair (cortisol, testosterone, progesterone, total and free thyroxine, total and free triiodothyronine, and thyroid-stimulating hormone) will be investigated.

Work Package 2. Food and Health (leader Antonija Sulimanec) By additional sampling of food and analyses, not only of pesticides but also various metal(oid)s, encompassing the diet of female adolescents and the adult population of both genders, is planned to contribute to a better definition of the exposure of the Croatian population to major contaminants in food and drinking water. Poorly understood mechanisms of action and the degree of toxicity of endocrine disruptors will be investigated, as well as inter-individual differences in sensitivity to these toxins in the human population during puberty. The harmful effects of exposure to low doses of toxic chemical mixtures on human health will also be explored. We will continue with developing of the theoretical models for the evaluation of oxidation potentials and antioxidant activities of flavonoids.

Work Package 3. Human biomonitoring and (epi)genetic (leader Ankica Sekovanić) This work package aims to investigate the impact of daily environmental exposure to chemicals, dietary and lifestyle habits, and genetic factors on biomarkers of exposure, epigenetic and metabolomic changes, and the occurrence of clinically manifested health disorders in humans, including occupational diseases. The study will examine the following: 1) the influence of MT2A and MTHFR gene polymorphisms on biomarkers of metal exposure and biochemical parameters (glucose, lipid, and nutritional status) in the general population (men and women) and/or in the vulnerable population (mother-newborn pairs); 2) the effects of TNF-α polymorphisms and chemical exposure on the occurrence of work-related skin disorders among hairdressing students; and 3) the prevalence of gene polymorphisms in proteins that are implicated in metabolism of xenobiotics (e.g., P-glycoprotein, cytochrome P450, glutathione-S-transferase) and their association with biomarkers of dietary pesticide exposure among adolescents.

Work Package 4. Mental Health (leader Adrijana Bjelajac) This work package aims to integrate insights into the relationships between sleep and daily functioning of different age groups exposed to different combinations of harmful environmental influences. In collaboration with work packages 2, 5, and 7, the knowledge gained from previous studies on sleep quality and daily functioning of different age groups (adolescents, adults, the elderly) in the context of exposure to harmful chemical and psychosocial factors will be analyzed. Enzymatic reaction mechanisms of tyrosinase at the molecular level will be identified by combining molecular modeling methods with experimental data from the literature.

Work Package 5. Occupational Safety and Health (leader Željka Babić) In this work package, research will be conducted to improve the prevention of occupational skin diseases in high-risk populations of hairdressing students and students for the profession of medical nurse/technician who are regularly exposed to a range of chemicals with harmful effects on the skin during practical training. Research will be conducted on three levels: 1) knowledge and application of occupational safety measures and other measures for the prevention of skin disorders during the vocational education of Croatian hairdressing students (use of protective gloves, method and frequency of hand skin washing and care); 2) through the design of a prospective cohort study, the predictive value of parameters of skin barrier function for the early detection of the development of inflammatory skin disorders related to work will be examined, contributing to primary and secondary prevention of occupational skin diseases; skin barrier function will be examined by non-invasive methods of measuring transepidermal water loss (TEWL), skin pH, hydration, and temperature; 3) based on the results of the above research, a preliminary educational intervention study will be conducted, including lectures, written popular materials, and gift packages for students and/or workers in selected schools and/or workplaces in the Republic of Croatia.

Work Package 6. Noise (leader Marija Kujundžić) This work package will investigate the habits of recreational headphone use in individuals aged 18 to 40. The sound intensity in headphones that respondents usually apply in quiet and noisy environments will be determined, hearing will be assessed by audiometry, and the association between headphone use and possible hearing damage will be evaluated. The innovation of the research is reflected in the assessment of noise exposure by direct measurement of noise exposure, i.e., measuring the sound volume applied by respondents in headphones, which will allow a better assessment of the relationship between exposure and health damage.

Work Package 7. Scientific Research Methodology (leader Jelena Kovačić) The goal of this research is to examine and propose methods for analyzing data from multiple sources (such as data generated within the framework of other work packages, data from the literature, including open-access data and summary data from publications, in silico data on the structure and physicochemical properties of pollutants, and freely available data used for regulatory purposes) in assessing exposure and adverse effects of chemicals. Statistical models and machine learning models allowing the inclusion of different types of data in one model will be explored for data analysis, such as models used in meta-analyses, Bayesian models enabling the inclusion of expert knowledge and results from analyses from other sources in the form of model parameters, and probabilistic graphical models. In collaboration with work packages 2, 4, and 5, the features of epidemiological self-assessment methods (questionnaires) will be evaluated as alternatives to objective measures (e.g., clinical diagnosis of diseases or measurements of biomarkers as indicators of exposure) due to the simplicity of their application, especially in situations where implementing more objective measures would be financially, temporally, or logistically challenging.

 

Exposure to pyrethroid and organophosphate insecticides in children – risk assessment for adverse effects on neuropsychological development and hormonal status (PyrOPECh)

Croatian Science Foundation research project
IP-2019-04-7193
web page: https://pyropech.imi.hr/
Participants: Jelena Kovačić, Adrijana Bjelajac, Jasminka Bobić (do 2022), Selma Cvijetić Avdagić, Jelena Macan, Rajka Turk, Martina Piasek (do 2020), Željka Babić, Jim Garvey, Marija Posavec, Vera Musil, Bernarda Krnić, Marijana Matek Sarić, Irena Keser, Saša Kralik Oguić, Iva Bebek, Gordana Jurak, Tatjana Petričević-Vidović, Anne Nugent, Matijana Jergović, Romana Gjergja Juraški, Snježana Sekušak-Galešev, Breige McNulty, Katja Dumić Kubat, Brendan Murray, Andreja Jurič, Marija Macan, Antonija Sulimanec Grgec, Patricia Tomac, Eva Anđela Delale
Head: Veda Marija Varnai
Summary
Epidemiological studies and animal experiments indicate the potential adverse effects of exposure to pyrethroid (PYR) and organophosphate (OP) insecticides on brain and sexual development in children, even at very low exposure levels. However, limited number of studies explored these potential risks during puberty, with inconsistent results and significant methodological limitations, such as insufficiently assessed exposure and cross-sectional study design. The main objective of the proposed research is to assess the risk of PYR and OP insecticide exposure to neuropsychological development and hormonal status in prepubertal and pubertal boys in a 2-year cohort study, while controlling for potential confounders, and using only non-invasive methods. Children’s exposure to PYR and OP insecticides will be assessed by biomonitoring (analysing urinary pesticide metabolites) in the 5th and 7th grades of elementary school, and by dietary exposure assessment – 24-hour dietary recall on five occasions during 2-year follow-up. In the 5th and 7th grades, neuropsychological development will be evaluated by testing attention, memory and simple psychomotor response speed, and by assessing the characteristics of child’s sleep and behaviour. Pubertal development will be evaluated by clinical examination (according to Tanner and Prader), and urinary gonadotropins and salivary sex hormones (testosterone and dehydroepiandrosterone sulfate) measurements. The research is expected to 1) increase the knowledge on possible risks of PYR and OP insecticides’ exposure for neurodevelopment and hormonal status in pubertal boys; 2) help to recognise deficiencies and assess available methodology for evaluation of developmental neurotoxicity and endocrine disruption within the framework of regulatory toxicology; 3) improve insufficient methodology for exposure assessment of non-bioaccumulative pesticides; 4) contribute to the better characterisation of pesticide exposure in the Croatian population.

Application of hepatic 3D cell model (HepG2) for safety evaluation of newly synthesized oximes, antidotes in treatment of organophosphate poisoning (OximeSafe3D)

Croatian-Slovenian Bilateral Scientific Research Project

Leader from the Slovenian Institution:

Martina Štampar, National Institute of Biology (NIB), Ljubljana, Slovenija

Project participants (IMROH, Zagreb):

Josip Madunić, Maja Katalinić, Antonio Zandona, Ana-Marija Lulić, Dora Kolić

Project participants (NIB, Ljubljana):

Bojana Žegura, Alja Štern, Matjaž Novak, Sonja Žabkar, Anamarija Habič

Duration: 2023-2025

Development of Bioactive Molecules for Neurodegenerative Diseases Treatment – BioMol4ND

PUBLICATIONS

Articles in journals
  1. Matošević, A Knežević, A. Zandona, N. Maraković, Z. Kovarik, A. Bosak, Design, synthesis and biological evaluation of biscarbamates as potential selective butyrylcholinesterase inhibitors for the treatment of Alzheimer’s disease, Pharmaceuticals 2022, 15, 1220. doi:10.3390/ph15101220 (IF (2021) = 5,21; Q1)
  2. Komatović, A. Matošević, N. Terzić-Jovanović, S. Žunec, S. Šegan, M. Zlatović, N. Maraković, A. Bosak, D. Opsenica, 4-aminoquinoline-based adamantanes as potential anticholinesterase agents in symptomatic treatment of Alzheimer’s disease. Pharmaceutics 2022, 14, 1305. doi:10.3390/pharmaceutics14061305 (IF= 6,52 (2021); Q1)
  3. Matošević, A. Radman Kastelic, A. Mikelić, A. Zandona, M. Katalinić, I. Primožič, A. Bosak, T. Hrenar, Quinuclidine-based carbamates as potential CNS active compounds, Pharmaceutics, 13 (2021), 3; 420, 15 doi:10.3390/pharmaceutics13030420 (IF (2020) =6,52; Q1).
Participation at scientific meetings
  1. Bartolić, Evaluation of 4-aminoquinolines as potential MTDL ligands for the treatment of Alzheimer`s disease, Joint IUBMB/FEBS Advanced Lecture Course “Molecular targets for anti-aging interventions”, Spetses Island, Grčka, 26.9.-1.10.2022.
  2. Matošević, Design, synthesis and biological evaluation of biscarbamates as potentialselective butyrylcholinesterase inhibitors for the treatment of Alzheimer`s disease, Joint IUBMB/FEBS Advanced Lecture Course “Molecular targets for anti-aging interventions”, Spetses Island, Grčka, 26.9.-1.10.2022.
  3. Komatović, Synthesis and biological evaluation of 4-amino-7- chloroquinolines as novel reversible inhibitors of human cholinesterases, Congress of the Croatian Society of Biochemistry and Molecular Biology HDBMB22: From Science to Knowledge , Brela, Hrvatska 28.9.-1.10.2022.
  4. Bosak, Biscarbamates as cholinesterase inhibitors in the treatment of Alzheimer’s disease, 14th International Meeting on Cholinesterases and 8th International Conference on Paraoxonases, Bologna, Italija, 18.-21.9.2022.
  5. Bartolić, Evaluation of hydrazone-based compounds as potential drugs for treatment of Alzheimer’s disease, European School of Medicinal Chemistry- 41st Advanced Course of Medicinal Chemistry and “E. Duranti” Seminar for PhD Students , Urbino, Italija, 3.-7.7. 2022.
  6. Matošević, Design, synthesis and biological evaluation of cyclic biscarbamates as selective butyrylcholinesterase inhibitors in the treatment of Alzheimer`s disease, European School of Medicinal Chemistry- 41st Advanced Course of Medicinal Chemistry and “E. Duranti” Seminar for PhD Students , Urbino, Italija, 3.-7.7. 2022.
  7. Bartolić, Hydrazone-based compounds evaluated as inhibitors of human cholinesterases, 17th International Symposium on Cholinergic Mechanisms (ISCM2022), 17th International Symposium on Cholinergic Mechanisms (ISCM2022), Dubrovnik, 8.-12.5.2022.
  8. Bosak, Evaluation of 4-aminoquinolines as potential anticholinesterase agents in the treatment of Alzheimer`s disease, 17th International Symposium on Cholinergic Mechanisms (ISCM2022), Dubrovnik, 8.-12.5.2022.
  9. Matošević, Synthesis and biological evaluation of heterocyclic meta-biscarbamates, 17th International Symposium on Cholinergic Mechanisms (ISCM2022), Dubrovnik, 8.-12.5.2022.
  10. Opsenica, A. Bosak, A. Matošević, K. Komatović, N. Maraković. 4-aminoquinolines, a privileged pharmacophore- from antimalarials to inhibition of cholinesterase. Book of Abstracts of the 27th Croatian Meeting of Chemists and Chemical Engineers (27HSKIKI). Veli Lošinj, Hrvatska. 5.-8.10.2021. (abstract)
  11. Matošević, A. Bosak. Application of NMR for monitoring the synthetic route and characterization of bisacrbamates. Book of abstracts of the Adriatic NMR Conference 2021. Primošten, Hrvatska. 13.-15.9.2021.; str. 46. (poster, abstract)
  12. Komatović, A. Matošević, N. Terzić- Jovanović, A. Bosak, D. Opsenica. Adamantane based derivatives as reversible inhibitors of human AChE and BChE. FEBS Open Bio 11 (Suppl. 1); 45th FEBS Congress, Molecules of Life: Towards New Horizons. Ljubljana, Slovenija. 3.-8. 7. 2021.; str. 213. (poster)
  13. Bosak, A. Matošević, A. Zandona, G. Šinko, K. Komatović, D. Opsenica. New 4-aminoquinoline-based cholinesterase inhibitors. FEBS Open Bio (Suppl. 1); 45th FEBS Congress, Molecules of Life: Towards New Horizons. Ljubljana, Slovenija. 3.–8. 7. 2021.; str. 310.-311. (poster, abstract)
  14. Matošević, A. Knežević, A. Bosak. Heterocyclic carbamates as potential drugs for the treatment of the neurodegenrative diseases. Book of abstracts of 29th European Colloquium on Heterocyclic Chemistry. Rouen, Francuska, 26-28. 4. 2021.; str. 143. (poster, sažetak)
  15. Matošević, A. Bosak. Design, synthesis and biological evaluation of compounds as active drugs in the central and peripheral nervous system. Knjiga sažetaka 5. Simpozija studenata doktorskih studija PMF-a, Zagreb, Hrvatska, 24-25. 4. 2021.; str. 146. (poster, abstract)
List of publications

BioMol4ND

Presentations
  1. Bosak, Development of bioactive molecules for treatment of Alzheimer’s diseases, Symposium „Cell-BasedResearch inToxicologyandDrug Design”, Zagreb, 26.1.2023.
  2. Matošević, Design and synthesis of selective butyrylcholinesterase inhibitors as multi- target directed ligands in the treatment of Alzheimer’s disease, 6th Mini Symposium of Section of Medicinal and Pharmaceutical Chemistry, Zagreb, 22.11.2022.
  3. Bosak, Biscarbamates as cholinesterase inhibitors in the treatment of Alzheimer’s disease, 14th International Meeting on Cholinesterases and 8th International Conference on Paraoxonases, Bologna, Italija, 18.-21.9.2022.
  4. Matošević, Design, synthesis and biological evaluation of biscarbamates as potential selective butyrylcholinesterase inhibitors for the treatment of Alzheimer`s disease, Joint IUBMB/FEBS Advanced Lecture Course “Molecular targets for anti-aging interventions”, Spetses Island, Grčka, 26.9.-1.10.2022.
  5. Bosak, Evaluation of 4-aminoquinolines as potential anticholinesterase agents in the treatment of Alzheimer`s disease, 17th International Symposium on Cholinergic Mechanisms (ISCM2022), Dubrovnik, 8.-12.5.2022.
  6. Matošević, Synthesis and biological evaluation of heterocyclic meta-biscarbamates, 17th International Symposium on Cholinergic Mechanisms (ISCM2022), Dubrovnik, 8.-12.5.2022.
  7. Opsenica, 4-aminoquinolines, a privileged pharmacophore – from antimalarials to inhibition of cholinesterase, The 27th Croatian Meeting of Chemists and Chemical Engineers (27HSKIKI), Veli Lošinj, 5.-8.10.2021.
  8. Matošević, Design, synthesis and biological evaluation of biscarbamates, Hotel Academia, Zagreb. 2.11.2021.
  9. Bosak, Razvoj lijekova za tretman neurodegegnarativnih bolesti. Županijsko stručno vijeće učitelja I nastavnika biologije Krapinsko-zagorske županije,1. 7. 2021.
  10. Matošević, Design, synthesis and biological evaluation of compounds as active drugs in the central and peripheral nervous system, 5th Symposium of PhD students, Faculty of science, Zagreb-a 24. 4. 2021.
Workshops/courses/webinars
  1. Bosak, A. Matošević; FDA Clinical Investigator Training Course, U. S. Food & Drug Administration, 7.-8.12.2022. (on-line)
  2. Bartolić, A. Bosak, A. Matošević; 1st Workshop in Mass Spectiometry in Life Sciences, Prirodoslovno-matematički fakultet, Zagreb, 24.-26.11.2022.
  3. Bartolić, A. Matošević; Uvod u molekulsko modeliranje, Institut “Ruđer Bošković”, Zagreb, 2.-6.12.2021.
  4. Bartolić, Education workshop – Better skills for better jobs: excellent lab book for an excellent career, 11.-12.11.2021. (on-line)
  5. Bosak; seminar Uvod u upravljanje projektima, Bicro Biocentar, Zagreb, 26. 10. 2021.
  6. Bartolić, A. Matošević; Practical workshop “ Tekućinska kromatografija i spektrometrija masa”, BICRO BIOCentar d.o.o., Zagreb 12.-13.10.2021.
  7. Matošević SPICA Virtual Session The importance of Chromatography in the Development of Vaccines, 14.10.2021. (on-line)
  8. Matošević; 2nd Symeres Precision Chemistry Conference on Tools for Molecular Recognition and Diagnostics, 12.-13.10.2021. (on-line)
  9. Matošević; Reaxys webinar, Hrvatsko kemijsko društvo i Elsevier, 23.04.2021.
  10. A. Matošević, webinar, Intact and peptide level characterization of nanobodies by high resolution HPLC-MS/MS, 12.2.2021.

Valentina Bušić, Marija Bartolić, Anita Bosak, Ana Matošević, Dajana Gašo Sokač (from the left) Symposium “Cell-Based Research inToxicology and Drug Design”, Zagreb, 26 Jan 2023

Ana Matošević, 6th Mini Symposium of Section of Medicinal and Pharmaceutical Chemistry, Zagreb

Ana Matošević (left) and M. Bartolić (right), Joint IUBMB/FEBS Advanced Lecture Course “Molecular targets for anti-aging interventions”, Spetses Island, Grčka

Marija Bartolić (left) and Ana Matošević (right), European School of Medicinal Chemistry – 41st Advanced Course of Medicinal Chemistry and “E. Duranti” Seminar for PhD Students , Urbino, Italija

Anita Bosak, 17th International Symposium on Cholinergic Mechanisms (ISCM2022), Dubrovnik

Ana Matošević, 17th International Symposium on Cholinergic Mechanisms (ISCM2022), Dubrovnik

Marija Bartolić, 17th International Symposium on Cholinergic Mechanisms (ISCM2022), Dubrovnik

Ana Matošević; predavanje na 5. Simpoziju studenata doktorskih studija PMF-a, Zagreb, Hrvatska

Ana Matošević; postersko priopćenje na Adriatic NMR Conference 2021. Primošten, Hrvatska