Development, validation and application of analytical methods for PBDE determination – DeValApp

Croatian Science Foundation

Project head: Darija Klinčić

Duration: 1 Oct 2018 – 30 Sep 2023

Funding: 1,424,500.00 HRK

Summary
The purpose of the proposed project is to develop and validate analytical methods for determining specific polybrominated diphenyl ether (PBDE) congeners in complex samples of house dust and human milk. PBDEs are widespread in the environment, they accumulate in the food chain and enter human organism where they can exert a number of negative impacts. House dust is recognized as an important source of exposure to PBDEs because of their intensive use as flame retardants in objects widely used in households (furniture, textiles, electrical and electronic devices). On the other hand, human milk is a reliable bioindicator of people’s exposure to these compounds. The development of analytical methods will involve the examination and comparison of various types of extraction techniques, whereby along with the solid phase extraction and ultrasonic extraction, particular emphasis will be put on the application of an unused technique, microwave extraction. Techniques and conditions for the cleanup of the extract from the interfering compounds and matrix components before the final instrument analysis will be investigated. The sensitivity and selectivity of gas-chromatographic determination of PBDEs by electron capture detectors, mass spectrometer and coupled tandem mass spectrometry will be determined. Validated methods will be confirmed by analysis of certified reference samples. During the project, house dust will be sampled in the homes of milk donors. The optimized and validated analytical methods will be used for PBDE analysis in collected samples. Results on PBDE levels and distribution will be the first of a kind in Croatia and will be used to assess the importance of PBDE intake in the body by inhaling house dust and exposure of general population to PBDEs. From all of the above mentioned, it will be concluded whether there is a potential risk to human health with a special emphasis on sensitive population groups of nursing mothers, infants and young children.