Mirta Milić

Mutagenesis Unit

PhD / Senior Scientific Associate

Place and date of birth

Zagreb, 24 August 1980

Education

  • 1 Mar 2013 – 31 Dec 2014 – Rome, Italy, IRCCS San Raffaele Pisana, Clinical and Molecular Epidemiology: POSTDOC FELLOWSHIP: automated micronucleus cytome assay and comet assay, SNP polymorphisms, finding and developing new biomarkers (in toxicology) of exposure to asbestos, finding sensitive biomarkers of exposure to low doses of ionising radiation, validation and standardisation of human exfoliated buccal micronucleus assay, flow cytometry
  • 16 Jul 2010 – Zagreb, Croatia, Faculty of Science: PhD, Ph. D. thesis:The significance of individual genome sensitivity in risk assessment of chronic professional exposure to low doses of ionising radiation, Supervisor: Ružica Rozgaj, PhD.
  • 21 Feb – 30 Aug 2009 – Alma Mater Studiorum, Faculty of Pharmacy, Bologna, Italy: SNP polymorphisms in DNA repair human systems
  • 2004-2010 – Zagreb, Croatia, Faculty of Science and Institute for Medical Research and Occupational Health, Molecular Biology, PhD student, assistant
  • 1998-2004 – Zagreb, Croatia, Faculty of Science, Division of Biology, Graduate Programme of molecular biology: molecular biologist, B. Sc. thesis: Assessment of bleomycin and mitomycin C genotoxicity using the comet assay, Supervisor: Nevenka Kopjar, PhD.

Work Experience

  • 2017- Institute for Medical Research and Occupational Health, Mutagenesis unit, Sen. Sci. Assoc., in the field: Eco-genetic research and human biomonitoring
  • 2013-2014 – IRCCS San Raffaele Pisana, Clinical and Molecular Epidemiology, Rome, Italy,Guest Researcher
  • 2011-2017- Institute for Medical Research and Occupational Health, Mutagenesis unit, Sci. Assoc., in the field: Genotoxicology and genetic monitoring
  • 2010-2011- Institute for Medical Research and Occupational Health, Mutagenesis unit, Research Assistant in the field: Genotoxicology
  • 2009-2009- Alma Mater Studiorum, Faculty of Pharmacy, Bologna, Italy, Guest Researcher
  • 2004-2010- Institute for Medical Research and Occupational Health, Mutagenesis unit, PhD student, Assistant, in the field: Genotoxicology

Fields of interest

human biomonitoring, genotoxicology, nanotoxicology, low doses of ionizing radiation, mechanism of DNA damage and repair, interindividual differences, comet assay in vitro, in vivo, in buccal cells, micronucleus assay in vitro, in vivo, in buccal cells, chromosomal aberrations, pesticide exposure, occupational exposure

Research and projects:

  • MC member and WG1 leader of COST PROJECT COST CA 15132 by prof Andrew Collins (Main proposer) title: The comet assay as a human biomonitoring tool (2016-2020)
  • INVESTIGATOR ON THE BILATERAL PROJECT BETWEEN CROATIA AND AUSTRIA: bilateral Austrian-Croatian project HR 15/2018: Pharmacokinetic profile of silver nanoparticles-the role of biological barriers (2018-2020), by IVANA VINKOVIĆ VRČEK and EVA ROBLEGG
  • LEADER AND INVESTIGATOR ON THE BILATERAL PROJECT BETWEEN CROATIA AND AUSTRIA: bilateral Austrian-Croatian project HR 05/2016: Possible early non-invasive biomarkers of chronic exposure to arsenic, by MIRTA MILIĆ and WALTER GOESSLER
  • INVESTIGATOR ON THE PROJECT OF I.VINKOVIĆ VRČEK, PhD: Grant of Croatian Science Foundation HrZZ IP-06-2016 “Interaction of metallic nanoparticlels with sulfur-rich biomolecules – implications for nano-bio interfaces NanoFaceS” (2017-2021)
  • INVESTIGATOR ON THE PROJECT OF RAPHAEL VALENCIA, PhD: Grant project of Mexican ministery PROFEPA: El proyecto general es evaluación del riesgo genotóxico por exposición a contaminantes ambientales (2016-2020)
  • INVESTIGATOR ON THE BILATERAL GERMAN CROATIAN PROJECT CRODIAS (2015-2016)
  • INVESTIGATOR ON THE PROJECT OF D. ZELJEZIC, PhD: Grant of Croatian Science Foundation “Organic Pollutants in Environment – Markers and Biomarkers of Toxicity OPENTOX” (2014-2018)
  • INVESTIGATOR ON THE PROJECT OF V.KASUBA,PhD: Grant of Ministry of Science, Education And Sports Of The Republic Of Croatia No.022-0222148-2137 “Genotoxicity of chemical and physical agents of natural and anthropogenic origin” (2007-2014)
  • INVESTIGATOR ON THE BILATERAL PROJECT BETWEEN CROATIA AND AUSTRIA No. WTZ-Hr04/2012.
  • INVESTIGATOR IN THE INAIL PROJECT (2013,2014): “Valutazione di biomarker di instabilità genetica, danno ossidativo e lesioni precoci in sangue, cellule esfoliate dalla mucosa boccale ed esalato bronchiale di lavoratori a rischio-amianto”, leader prof STEFANO BONASSI
  • INVESTIGATOR ON THE AIRC PROJECT (2014) (Associazione Italiana per la Ricerca sul Cancro): “Micronucleus assay in buccal exfoliated cells to measure DNA damage and predict cancer in healthy subjects”,  leader prof STEFANO BONASSI
  • PhD STUDENT ON THE PROJECT OF V. KAŠUBA, PhD: Grant of Ministry of Science, Education And Sports Of The Republic Of Croatia “Genotoxicity of natural and anthropogenic agents”

Additional training

Training as a trainee:

  • 11.02.-22.02.2019 – STSM- Short Term scientific mission Cost Action, Mol, Belgium and Maastricht, Netherlands and VITO, Belgium, Mol and PHARTOX, Medical University, Maastricht, Netherlands
  • 18-19.12.2018- Adverse outcome pathways (AOP): the How and Why in Development and Application, Unity Through Knowledge Fund and University of Zagreb, Faculty of Science, Department of Biology, Zagreb, Croatia
  • 5-9.11.2018- REGULATORY TOXICOLOGY COURSE, EUROTOX and SLOVENIAN SOCIETY OF TOXICOLOGY , Otočec, Slovenia
  • 14.06.2018- STRATEGIC AWARNESS EVENT-SYSTEMS MEDICINE IN ITALY-WHO AND WHERE?PRESENT AND FUTURE PERSPECTIVES,Ministero della Salute, Direzione Generale Ricerca e Innovazione in Sanita, Rome, Italy
  • 23.05.-25.06.2018 – STSM- Short Term scientific mission Cost Action CA15132 hCOMET: pooled analysis of comet assay data in order to obtain definitive information on the reasons for inter-individual variation in levels of DNA damage, IRCCS San Raffaele Pisana, Rome, Italy
  • 09-10.05.2018- Flow cytometry workshop at MEDILS, Split, Croatia
  • 2018 – EUROTOX, IUTOX AND SCT, CONTINUING EDUCATION COURSE Water Security: Integrating Lessons Learned for Water Quality and Sustainability, Belgrade, Serbia
  • 01-03-2017 to 31-03-2017 STSM- Short Term scientific mission Cost Action CA15132 hCOMET: Adjusting collected data for the hCOMET database from different studies and laboratories all over the world and creation a Masterfile convenient for further statistical analysis; IRCCS San Raffaele Pisana; Rome, Italy
  • 2017- EUROTOX, CONTINUING EDUCATION COURSE ˝ Nanotoxicology and risk assessment: the state of the art ˝, Bratislava, Slovakia
  • 2016 – OBERSCHLEISSHEIM/ MUNICH, GERMANY; GERMAN FEDERAL OFFICE FOR RADIATION PROTECTION (BfS); Two week course InterRad- Interdisciplinary Radiation Research: Automated micronucleus assay, automated comet assay, automated chromosomal aberration assay-dicentric assay, epidemiological studies on radiation exposed workers
  • 2015 – qPCR, Zagreb, BIORAD and Labena
  • 2013, 2014, Rome, Italy, IRCCS San Raffaele Pisana, Clinical and Molecular Epidemiology: automated micronucleus assay, comet assay, γH2aX comet assay, SNP polymorphisms, discovering biomarkers for: alcoholics and head and neck cancer patients, people exposed to asbestos and with asbestosis, people exposed to low doses of ionising radiation, finding good biomarkers for migraine diagnosis, oxidative stress in COPD patients, validation and standardisation of micronucleus assay on human exfoliated buccal cells, flow cytometry
  • 2012 – Kemer, Antalya, Turkey: NANOLINEN, NEW INDIGO, NanoSafety Congress – Workshop IN THE SAFETY ASSESSMENT OF NANOMATERIALS: New Paradigms; -Workshop on GENOTOXICITY TESTS TO ASSESS HUMAN GENOTOXICITY
  • 11-15 Jul 2011, Zagreb, Croatia: SYSTEMCOM STATISTICA ADVANCED COURSE OF STATSOFT
  • 27 Jun-1 Jul 2011, Belgrade, Serbia, University of Belgrade, Faculty of Pharmacy: EUROTOX BASIC TOXICOLOGY COURSE
  • 2009 – Perugia, Italy, University of Perugia: 8th INTERNATIONAL COMET ASSAY WORKSHOP
  • 2009 – Bologna, Italy, Faculty of Pharmacy: 6 MONTHS OF LEARNING OF SNP POLYMORHISM METHODS AND DNA ISOLATION
  • 2008 – Utrecht, Netherland, IRAS (Institute for Risk Assessment Sciences): THE COURSE ON MOLECULAR EPIDEMIOLOGY OF CHRONIC DISEASES
  • 2006 – Tekirova, Antalya, Turkey: THE IXth INTERNATIONAL WORKSHOP ON RADIATION DAMAGE TO DNA
  • 2005 – Cracow, Poland, EUROTOX CONTINUING EDUCATION COURSE: ˝HIGH THROUGHPUT ASSAYS IN TOXICOLOGICAL TESTING˝

Training as a trainer:

  • ZAGREB, APRIL 4-8, 2016, EUROTOX –BASIC TOXICOLOGY COURSE 2016 “PRINCIPLES OF TOXICOLOGY”, LECTURE: Mutagenesis (Ksenija Durgo and Mirta Milić, Croatia)
  • 28.2-2.3.2018- 3 DAY COURSE ON STATISTICAL AND EPIDEMIOLOGICAL ISSUES IN HUMAN BIOMONITORING STUDIES: AN HCOMET COURSE, A JOINT HCOMET – UK MEG  INITIATIVE, LECTURE:PRACTICAL ISSUES (SPECIMEN COLLECTION AND STORAGE, TIMING, OPERATORS TRAINING)
  • 29.1.-1.2.2019 HCOMET COST ACTION CA15132 THEORETICAL AND PRACTICAL TRAINING COURSE ON THE USE OF NON-INVASIVE SAMPLED MATRIXES IN THE COMET ASSAY AS WELL AS DIFFERENT TYPES OF WHITE BLOOD CELLS, RIGA, LATVIA, UNIVERSITY OF LATVIA, HOUSE OF NATURE, 29.1-1.2.2019 AS A TRAINER WITH LECTURE:THE USE OF BUCCAL CELLS IN HUMAN BIOMONITORING AND EARLY DISEASE DETECTION.
  • AS AN INSTRUCTOR IN A HANDS-ON TRAINING: HANDS-ON TRAINING WITH BUCCAL CELLS.
  • 5-6.10.2019 – BASIC COMET ASSAY COURSE,; SARAJEVO
  • 16-18.10.2019, TURKEY- CEC 2019-10th International Congress of Turkish Society of Toxicology ,CEC (CONTINUOUS EDUCATIONAL COURSE) 2-RECENT DEVELOPMENTS AND THE USE OF NON-INVASIVE SAMPLES IN COMET ASSAY: THE USE OF BUCCAL CELLS IN HUMAN BIOMONITORING AND EARLY DISEASE

Mentorships:

  • 2019, 2020-mentor of the doctoral thesis of the PhD student from  Faculty of Food Technology and Biotechnology (Ivan Ožvald)
  • 2020-comentor of master disertation of the student from Faculty of Science, Department of Biology
  • (Dunja Šikić, ˝Effect of anaesthetic isoflurane and ionising radiation on primary DNA damage in mice˝)
  • 2019-comentor of master disertation of the student from  Faculty of Food Technology and Biotechnology (Tena Križmanić, ˝Protective effect of dandelion extracts on different human cell lines˝)
  • 2019-comentor of master disertation of the student from  Faculty of Food Technology and Biotechnology (Ivana Ledenko, ˝Protective effect of rosemary extracts on different human cell lines˝)
  • 2012.-co-mentor of master disertation of the student from  Faculty of Science, Department of Biology (Sanja Halovanić, ˝The combined effect of anaesthetic sevoflurane and ionising radiation on primary DNA damage in mice˝)
  • 2012.- mentor of master disertation from Faculty of Science, Department of Biology (Maja Heleš, ˝Assessment of primary DNA damage in blood and different organs in Swiss albino mices after exposure to anesthetic sevoflurane˝)
  • 2012.-mentor of master disertation from Faculty of Science, Department of Biology (Željka Modrić, ˝Assessment of primary DNA damage in blood and different organs in Swiss albino mices after exposure to ionising radiation˝)
  • 2011.- co-mentor of master disertation of the student from Faculty of pharmacy and biochemistry (Maja Kišan, ˝Oxidative damage of DNA in metabolic syndrome˝)

Select Publications

Memberships

  • 2016- MC member of COST Project CA 15132 hCOMET
  • 2015- member of Genetic Mexican Society
  • 2010- member of Croatian Radiation Protection Association
  • 2010- member of Croatian Association For Biochemistry And Molecular Biology
  • 2004- member of Croatian Society of Toxicology
  • 2004- member of Croatian Genetic Society

Other

Personal interests and hobbies:
singing, crochet, cooking

List of publications