Tamara Zorbaz

Biochemistry and Organic Analytical Chemistry Unit

Master of Medical Biochemistry / Postdoctoral Researcher

Place and date of birth

Zagreb, 2 July 1990

Education

  • 2015-2018: Faculty of Science, University of Zagreb Study: Postgraduate doctoral study in Chemistry, Biochemistry Supervisor: Dr Zrinka Kovarik
  • 2009-2014: Faculty of Pharmacy and Medical Biochemistry, University of Zagreb Study: Master in Medical Biochemistry and Laboratory Medicine Thesis: “The effect of Hsp90 inhibitor, 17-DMAG-a, on the expression of galectin-3”, under the supervision of Professor Jerka Dumić
  • 2005-2009: Gimnazija Lucijana Vranjanina

Work Experience

  • Institute for Medical Research and Occupational HeaIth (IMROH), Zagreb, Croatia: Research Assistant (2015-2018), Postdocotral Researcher (2019-)
  • Cognition and Action Group (COGNAC G), Le Centre national de la recherche scientifique (CNRS MD, UMR 8257), Université Paris Descartes, Paris, France: Research stay (September-October 2016, 2 mo)
  • Department of Biochemistry and Molecular Biology, Faculty of Pharmacy and Biochemistry, University of Zagreb, Croatia: Master’s degree thesis research and volunteering (April-November 2014)

Fields of interest

Mechanisms of interaction between cholinesterases and organophosphorus (OP) compounds, reactivation of OP-inhibited cholinesterases, biochemical and pharmacological evaluation of novel antidotes.

Additional training

COURSES/WORKSHOPS/TRAININGS:

  • Adverse outcome pathways (AOP): The how and why of development & application workshop (Faculty of Science, University of Zagreb, Croatia, December 2018)
  • Mendeley Reference Manager course (IMROH, Zagreb, Croatia, November 2018)
  • FEBS workshop on molecular life science education (University of Zagreb, Croatia, April 2018)
  • Communication skills (Faculty of Science, University of Zagreb, Croatia, June 2017)
  • Using 3R approach in the work with laboratory animals (CroLASA i HIIM; Zagreb, Croatia, October 2015)
  • Managing the knowledge, intellectual property, transfer of the technology and innovations workshop (Faculty of Science, University of Zagreb, Croatia, July 2015)
  • Symposium and workshop on microscale thermophoresis (IntegraLife FP7 RegPot, University of Zagreb, Croatia, July 2015)
  • The InnoMol molecular interactions Workshop (Ruđer Bošković Institute, Zagreb, Croatia, June 2015)
  • Skeletal muscle research from cell to human Workshop (Faculty of Medicine, University of Ljubljana, Slovenia, May 2015)
  • Novel diagnostic and therapeutic approach in complex genetic disease, International CEEPUS Summer School (Portorož, Slovenia, August 2014)
  • Parathyroid  multidisciplinary approach to diagnostics and monitoring of surgery and therapy success training course (Faculty of the Pharmacy and Biochemistry, University of Zagreb, Croatia, June 2013)

Awards

  • Travel Award for XVIth International Symposium on Cholinergic Mechanisms, Rehovot, Israel (2019)
  • Poster Award at the HDBMB2019 Congress Crossroads in Life Sciences, Lovran, Croatia (2019)
  • Travel fellowship for 13th International Meeting on Cholinesterases and the 7th International Conference on Paraoxonases, Hradec Králové, Czech Republic (2018)
  • Poster Award at the 13th International Meeting on Cholinesterases and the 7th International Conference on Paraoxonases, Hradec Králové, Czech Republic (2018)
  • FEBS Junior travel fellowship for FEBS3+ conference From molecules to living systems, Siófok, Hungary (2018)
  • YSF grant for participation at the 17th YSF and 42nd FEBS Congress, Jerusalem, Israel (2017)
  • Poster Award at the 16th Medical Chemical Defence Conference, Munich, Germany (2017)
  • ISN/IBRO-PERC Young Investigator Poster Prize, XVth International Symposium on Cholinergic Mechanisms, Marseille, France (2016)
  • Scholarship of the French Government for a short-term professional development (2016)

Select Publications

  1. T. Zorbaz, P. Mišetić, N. Probst, S. Žunec, A. Zandona, G. Mendaš, V. Micek, N. Maček Hrvat, M. Katalinić, A. Braïki, L. Jean, P.-Y. Renard,V. Gabelica Marković, Z. Kovarik. Pharmacokinetic evaluation of brain penetrating morpholine-3-hydroxy-2-pyridine oxime as an antidote for nerve agent poisoning. ACS Chemical Neuroscience, 2020, accepted. https://pubs.acs.org/doi/10.1021/acschemneuro.0c00032
  2. T. Zorbaz, A. Braïki, N. Maraković, J. Renou, E. de la Mora, N. Maček Hrvat, M. Katalinić, I. Silman, J. L. Sussman, G. Mercey, C. Gomez, R. Mougeot, B. Pérez, R. Baati, F. Nachon, M. Weik, L. Jean, Z. Kovarik, P.-Y. Renard. Potent 3-Hydroxy-2-Pyridine Aldoxime Reactivators of Organophosphate-Inhibited Cholinesterases with Predicted Blood-Brain Barrier Penetration. Chemistry – A European Journal, 2018, 24, 9675-91. https://onlinelibrary.wiley.com/doi/abs/10.1002/chem.201801394
  3. T. Zorbaz, D. Malinak, N. Maraković, N. Maček Hrvat, A. Zandona, M. Novotny, A. Skarka, R. Andrys, N. Benkova, O. Soukup, M. Katalinić, K. Kuca, Z. Kovarik, K. Musilek. Pyridinium Oximes with Ortho-Positioned Chlorine Moiety Exhibit Improved Physicochemical Properties and Efficient Reactivation of Human Acetylcholinesterase Inhibited by Several Nerve Agents. Journal of Medicinal Chemistry, 2018, 61, 10753-66. https://pubs.acs.org/doi/abs/10.1021/acs.jmedchem.8b01398?journalCode=jmcmar
  4. T. Zorbaz, D. Malinak, K. Kuca, K. Musilek, Z. Kovarik. Butyrylcholinesterase inhibited by nerve agents is efficiently reactivated with chlorinated pyridinium oximes. Chemico-Biological Interactions, 2019, 307, 16-20. https://www.sciencedirect.com/science/article/abs/pii/S0009279719303941?via%3Dihub
  5. N. Maček Hrvat, T. Zorbaz, G. Šinko, Z. Kovarik. The estimation of oxime efficiency is affected by the experimental design of phosphylated acetylcholinesterase reactivation. Toxicology Letters, 2018, 293, 222-228. https://www.sciencedirect.com/science/article/pii/S0378427417314819?via%3Dihub
  6. B. L. d’Incamps, T. Zorbaz, D. Dingova, E. Krejci, P. Ascher. Stoichiometry of the heteromeric nicotinic receptors of the Renshaw cell. The Journal of Neuroscience, 2018, 38 (21), 4943-4956 http://www.jneurosci.org/content/38/21/4943

Memberships

  • Croatian Society of Biochemistry and Molecular Biology (HDBMB)
  • The International Society for Neurochemistry (ISN)

List of publications