Vilena Kašuba

Samobor, 19 October 1960

• 1980-1985 Faculty of natural sciences University of Zagreb, Croatia, Biology, Experimental biology
• 1985-1989 Faculty of natural sciences, University of Zagreb, Croatia Biology
• 1989-1995 doctoral dissertation in Mutagenesis Unit, Institute for Medical Research and Occupational health, Zagreb, Croatia Mentor: Dr.sc. Đurđa Horvat

QUALIFYING WORK

• Thesis: Kašuba V.: Distribution and frequency of gastrin cells under experimental conditions in the Fischer rats[thesis]. Zagreb: Faculty of Science, University of Zagreb; 1985 Mentor: N. Kralj-Klobučar.
• Thesis: Kašuba V .: Immunochemical reactivity of some human malignant tumor and healthy tissue. Master’s thesis. Faculty of Science, University of Zagreb in 1985. Mentor: Prof.dr. Terezija Hrženjak
• Dissertation: Kašuba V .: The genotoxic effect of 131J on human lymphocytes. Doctoral dissertation, Faculty of Science, University of Zagreb, Zagreb 1995. Mentor: Dr.sc. Đurđa Horvat

1985-1989.y. – Veterinary faculty Universitiy of zagreb, Croatia, Biology department

– from 1989.y. Mutagenesis Unit, Institute for Medical Research and Occupational Health, Zagreb, Croatia

Participation in scientific research topics, projects and programs:

– From 1985. Associate on the topic “The interaction of glycoconjugates and biological status of the organism” (1.08.11.00.03) within the “Differentiated cells in vitro – plant-specific macromolecules, bioprosthesis and models for biological tests (UE 9) (Funding Source: Federal Committee for Science , technology and information; coordinator: INEP, Zemun), led by Prof. dr. Terezija I. Hrženjak (Department of Biology, Faculty of Veterinary Medicine, University of Zagreb)

-From 1989 to 1993. – Associate on the technical project with the IAEA (International Agency for Radiation Protection) and the Ministry of Economy of the Republic of Croatian (CRO / 9/002) “The development of infrastructure for radiation protection and nuclear safety in the Republic of Croatia)

– From 1993-1996. Associate on the topic “The target sites in the human genome associated with the process of carcinogenesis (1-08-329) – this project was included in the program of international cooperation COST 244 Project-” Biomedical effects of electromagnetic radiation ”

– From 1998-2002.godine – Associate on the project “Eco-genetic research activities mutagenic living and working environment with special reference to a specific cell response” (00220107)

– Since 2002 to 2006 Head of the project “Genotoxicity of natural and anthropogenic agents” (0022019) funded by the Ministry of Science, Education and Sport, Republic of Croatia

– From 2005 to 2006 Associate on the technical project “Application of macromolecular formulation (G-90) from earthworm Eisenia foetida tissues in biomedicine” (TP-03 / 009,841), led by Dr. M. Grdiša with RBI; project is financed by the Ministry of Science, Education and Sport, Republic of Croatia

– From 2007 to 2013. Head of the “Genotoxicity of chemical and physical agents of natural and anthropogenic origin” (022-0222148-2137), and under the program 0222148- “Experimental toxicology of natural and synthetic substances”, financed by the Ministry of Science, Education and Sport, Republic of Croatia

-From 2011-2013 – Associate on the project: “Monitoring of organic and inorganic pollutants in the environment Plitvice Lakes National Park” (2011-2013) led by Ph.D. Snjezana Herceg Romanic

– 2014 g. to 2018.g.- project associate OPENTOX – HRZZ s (Croatian Science Foundation) – led by- Ph.D. Davor Želježić.

– Mechanisms of action and effects of the genome of different genotoxic agents, anesthetics, cytostatics, heavy metals (cadmium, lead, mercury, …), dental composites and root fillings, and ionizing radiation at the cellular level;

– The effects of long term exposure to genotoxic agents at occupationally exposed populations

As part of the technical project “Development of infrastructure protection Radiation and Nuclear Safety in the Republic of Croatia,” the Ministry of Economy of the Republic of Croatian and the IAEA stayed alone at the National Institute for Radiobiology and radiohygene Frederic Joliot Curie in Budapest, Hungary (April-July 1994) .

Rector’s Award, Zagreb 1985: In the fourth year of study, for a seminar entitled: “Stereologic processing enteroendocrine cells in rats with transplanted tumor of the pituitary gland” Supervisor: Ph.D. sc. Nada Kralj-Klobučar

Hrženjak T, Popara T, Kašuba V, Roguljić A. Ugljikohidratne determinante u malignim tumorima – komparativni prikaz. Veterinarski arhiv 1986; 56(5): 239-245.

Hrženjak T, Đelajlija-Kostić M, Šerman D, Gerenčer M, Kašuba V. Lectins isolated from the trout (Salmo trutta fario) mature spawn. Iugoslav Physiol Pharmacol Acta 1988; 24(3):313-323.

Hrženjak T, Vodopija I, Karlović M, Kašuba V, Đelajlija-Kostić, Gojšić S. Structural similarity of fox brain glycolipids and rabies virus glycoproteins. Period Biol 1989; 91(1): 199-204.

Hrženjak T, Roguljić A, Tiška-Rudman Lj, Kašuba V. The role of «MN» eythrocytic antigens in breast carcinoma. PeriodBiol 1991; 93(2): 175-178.

Hrženjak T, Kašuba V, Roguljić A, Pišl M, Pende B, Buneta L, Vladović-Relja T, Muić V, Gerenčer M. Carcinoembrionic antigen (CEA): receptor for effector molecules? Iugoslav Phisiol Pharmaceutica Acta 1991; 27(1): 1-14.

Hrženjak T, Roguljić A, Tiška-Rudman Lj, Kašuba V. Uloga “MN” eritrocitnih antigena i sijalinske kiseline u karcinoma dojke. Libri Oncol 1991; 20(1-2): 21-26.

Hrženjak T, Hrženjak M, Kašuba V, Effenberger-Marinculić P, Levanat S. A new source of biologically active compounds-earthworm tissue (Eisenia foetida, lumbricus rubelus). Comp Biochem Physiol 1992; 102 A (3):441-447.

Hrženjak T, Roguljić A, Kašuba V, Karlović M. The role of sialic acid and Ca++ ions in rabies virus-infected foxes brain. Comp Immun Microbiol Infect Dis 1994; 17(1): 71-75.

Kašuba V, Šentija K, Garaj-Vrhovac V, Fučić A. Chromosome aberration in peripheral blood lymphocytes from a control individuals. Mutation Res 1995; 364(4):187-193.

Kašuba V, Šentija K, Rozgaj R. Persistence of unstable chromosome aberrations in medical stuff occupationally exposed to ionizing radiation. Ninth International Congress of the International Radiation Protection Association,Vienna, Austria, April 14-19, 1996. Proceedings. Vol. 3. p. 61-63.

Kašuba V. Biološki učinci radionuklida joda-131. Arh Hig Rada Toksikol 1997; 48: 247-257.

Kašuba V, Rozgaj R, Šentija K. Chromosomal Aberrations in Medical Staff Occupationally Exposed to X-rays. Follow-up Study. Arch Ind Hyg Toxicol 1998; 49(1):1-8.

Rozgaj R, Kašuba V, Perić M. Chromosome aberrations in operating room personnel. Am J Ind Med 1999; 35: 642-646.

Rozgaj R, Kašuba V, Šentija K, Prlić I. Radiation-induced chromosomal aberrations and haematological alterations in hospital workers. Occup Med 1999; 49: 353-360.

Kašuba V, Rozgaj R, Garaj-Vrhovac V. Analysis of sister chromatid exchange and micronuclei in peripheral lymphocytes of nurses handling cytostatic drugs. J Appl Toxicol 1999; 19: 401-404.

Kašuba V, Kusić Z, Garaj-Vrhovac V. Micronuclei in cytokinesis-blocked lymphocytes of patients following iodine-131 radiotherapy. Radiol Oncol 1999; 33(1): 35-41.

Rozgaj R, Kašuba V. Chromosome aberrations and micronucleus frequency in anaesthesiology personnel. Arh Hig Rada Toksikol 2000; 51: 361-368.

Kašuba V, Rozgaj R. Biological effects of cadmium. Period Biol 2000; 102: 365-371.

Kašuba V, Rozgaj R, Šentija K. Cytogenetic changes in subjects occupationally exposed to benzene. Chemosphere 2000; 40: 307-310.

Rozgaj R, Kašuba V, Jazbec A. Preliminary study of cytogenetic damage in personnel exposed to anesthetic gases. Mutagenesis 2001; 16: 139-143.

Rozgaj R, Kašuba V, Šimić D. The frequency of dicentrics and acentrics and the incidence of rogue cells in radiation workers. Mutagenesis 2002; 17: 135-139.

Kašuba V, Rozgaj R, Kopjar N, Želježić D, Jazbec A. Sister chromatid exchange analysis in peripheral blood lymphocytes of healthy individuals. Biologia, Bratislava 2002; 57: 401-407.

Trošić I, Bušljeta I, Kašuba V, Rozgaj R. Micronucleus induction after whole-body microwave irradiation of rats. Mutat Res 2002; 521: 73-79.

Kašuba V, Rozgaj R. Micronucleus distribution in human peripheral blood lymphocyte treated in vitro with cadmium chloride in G0 and S phase of the cell cycle. Chemosphere 2002; 49: 91-95.

Rozgaj R, Kašuba V, Fučić A. Genotoxicity of cadmium chloride in human lymphocytes evaluated by the comet assay and cytogenetic tests. J Trace Elem Med Biol 2002; 16: 187-192.

Kašuba V, Rozgaj R, Matek-Šarić M, Blanuša M. Evaluation of genotoxic damage of cadmium chloride in peripheral blood of suckling Wistar rats. J Appl Toxicol 2002; 22: 271-277.

Kašuba V, Rozgaj R, Fučić A, Varnai VM, Piasek M. Lead acetate genotoxicity in suckling rats Biologia 2004; 59: 779-785.

Rozgaj R, Kašuba V, Blanuša M. Mercury chloride genotoxicity in rats following oral exposure, evaluated by comet assay and micronucleus test. Arh Hig Rada Toksikol 2005; 56: 9-15.

Fučić A, Želježić D, Kašuba V, Kopjar N, Rozgaj R, Lasan R, Mijić A, Hitrec V, Lucas JN. Stable and Unstable Chromosome Aberrations Measured after Occupational Exposure to Ionizing Radiation and Ultrasound. Croat Med J 2007; 48:371-377.

Milić M, Kašuba V, Oreščanin V, Želježić D, Kopjar N, Rozgaj R. Chromosome damage in workers in cigarette manufacturing industry. J Appl Tox 2008; 28(3): 399-404.

Kašuba V, Rozgaj R, Jazbec A. Chromosome aberrations in peripheral blood lymphocytes of Croatian hospital staff occupationally exposed to low levels of ionizing radiation. Arh Hig Rada Toksikol 2008; 59: 251-259.

Rozgaj R, Kašuba V, Brozović G, Jazbec A. Genotoxic effects of anaesthetics in operating theatre personnel evaluated by the comet assay and micronucleus test. Int. J Hyg Environ Health 2009; 212:11–17.

Kopjar N, Garaj-Vrhovac V, Kašuba V, Rozgaj R, Ramić S, Pavlica V, Želježić D. Assessment of genotoxic risks in Croatian health care workers occupationally exposed to cytotoxic drugs: A multi-biomarker approach. Int J Hyg Environ Health 2009; 212 : 414–431.

Brzović V, Miletić I, Želježić D,Mladinić M, Kasuba V, Ramić S, Anić I. In vitro genotoxicity of root canal sealers. International Endodontic Journal, 2009; 42: 253–263.

Kopjar N, Kašuba V, Rozgaj R, Želježić D, Milić M, Ramić S, Pavlica V, Milković-Kraus S. The genotoxic risk in health care workers occupationnally exposed to cytotoxic drugs. A comprehensive evaluation by the SCE assay [Review]. J Environ Sci Health Part A-Toxic/ Hazardous Substances & Environmental Engineering. 2009; 44(5):462-479.

Kašuba V, Rozgaj R, Milić M, Želježić D, Kopjar N, Pizent A, Kljaković-Gašpić Z. Evaluation of lead exposure in battery-manufacturing workers with focus on different biomarkers. J Appl Toxicol 2010; 30(4):321-328.

Mataušić-Pišl M, Čupić H, Kašuba V, Mikecin AM, Grdiša M. Tissue extract from Eisenia foetida as a wound-healing agent. Eur. Rev. Med. Pharmacol. Sci. 2010; 14(3):177-184.

Brozović G, Oršolić N, Rozgaj R, Kašuba V, Knežević F, Knežević AH, Benković V, Lisičić D, Borojević N, Đikić D. DNA damage and repair after exposure to sevoflurane in vivo, evaluated in Swiss albino mice by the alkaline comet assay and micronucleus test. J Appl Genet 2010;51(1):1-8.

Milić M, Rozgaj R, Kašuba V, Oreščanin V, Balija M, Jukić I. Correlation between folate and vitamin B12 and markers of DNA stability in healthy volunteers: preliminary results. Acta Biochimica Polonica 2010; 57(3): 339-345.

Šegvić-Klarić M, Daraboš D, Rozgaj R, Kašuba V, Pepeljnjak S. Beauvericin and ochratoxin A genotoxicity evaluated using the alkaline comet assay: single and combined genotoxic action. Arch Toxicol 2010; 84(8): 641-650.

Kašuba V, Rozgaj R, Gamulin M, Trošić I. Assessment of Cyto/Genotoxicity of Irinotecan in V79 Cells Using Comet, Micronucleus and Chromosomal Aberrations Assay. Arh Hig Rada Toksikol 2010; 61:1-9.

Kopjar N, Kašuba V, Milić M, Rozgaj R, Želježić D, Gajski G, Mladinić M, Garaj-Vrhovac V. Normal and cut off values of the cytokinesis-block micronucleus assay on peripheral blood lymphocytes in the Croatian general population. Arh Hig Rada Toksikol 2010; 61(2): 219-234.

Kopjar N, Želježić D, Kašuba V, Rozgaj R. Antineoplastic drugs as a potential risk factor in occupational settings: Mechanisms of action at the cell level, genotoxic effects, and their detection using different biomarkers. Arhiv Hig Rada Toksikol 2010; 61(1): 121-146.

Milić M, Rozgaj R, Kašuba V, Kubelka D, Angelini S, Hrelia P. Povezanost polimorfizama gena za popravak DNA I osjetljivost na ionizirajuće zračenje. Arh Hig Rada Toksikol 2010; 61 Suppl.:107-121.

Kašuba V, Rozgaj R, Milić M, Želježić D, Kopjar N, Pizent A, Kljaković-Gašpić Z, Jazbec A.Evaluation of genotoxic effects of lead in pottery-glaze workers using micronucleus assay, alkaline comet assay and DNA diffusion assay. Int Arch Occupat Environ Health 2012; 85:807-18.

Kašuba V. Gujavica – izvor biološki aktivnih molekula, lijek I hrana. Farm Glas 2012;68:401-15.

Klarić E, Par M, Profeta I, Kopjar N, Rozgaj R, Kašuba V, Želježić D, Tarle Z. Genotoxic effect of two bleaching agents on oral mucosa. Cancer Genom Proteom 2013; 10(5): 209-215.

Galić E, Tadin A, Galić N, Kašuba V, Mladinić M, Rozgaj R, Biočina-Lukenda D, Galić I, Želježić D. Micronucleus, alkaline, and human 8-oxoguanine glycosylase 1 modified comet assays evaluation of glass-ionomer cements – in vitro. Arh Hig Rada Toksikol 2014; 65(2): 179-187.

Milić M, Rozgaj R, Kašuba V, Jazbec A-M, Starčević B, Lyzbicki B, Ravegnini G, Zenesini C, Musti M, Hrelia P, Angelini S. Polymprphisms in DNA repair genes: link with biomarkers of the CBMN cytome assay in hospital workers chronically exposed to low doses of ionising radiation. Arh Hig Rada Toksikol 2015; 66 (2): 109-120.

Žunec S, Kašuba V, Pavičić I, Marjanović AM, Tariba B, Milić M, Kopjar N, Pizent A, Lucić-Vrdoljak A, Rozgaj R, Želježić D. Assessment of oxidative stress responses and the cytotoxic and genotoxic potential of the herbicide tembotrione in HepG2 cells. Food and Chemical Toxicology 2016; 94: 64-74.

Želježić D, Mladinić M, Žunec S, Lucić-Vrdoljak A, Kašuba V, Tariba B, Živković T, Marjanović AM, Pavičić I, Milić M, Rozgaj R, Kopjar N. Cytotoxic, genotoxic and biochemical markers of insecticide toxicity evaluated in human peripheral blood lymphocytes and an HepG2 cell line. Food and Chemical Toxicoloy 2016; 96: 90 – 106.

Poglavlje u knjizi:

Selectic topics in DNA REPAIR, Ed.C.C. Chen, 2011

POGLAVLJE 19: MILIĆ M, ROZGAJ R, KAŠUBA V, JAZBEC AM, HRELIA P, ANGELINI S: THE INFLUENCE OF INDIVIDUAL GENOME SENSITIVITY IN DNA DAMAGE REPAIR ASSESSMENT IN CHRONIC PROFESSIONAL EXPOSURE TO LOW DOSES OF IONIZING RADIATION, RIJEKA, HRVATSKA, INTECH 2011. STR. 437-465.

Croatian Society of Toxicology

Participation in scientific research topics, projects and programs:

• From 1985. Associate on the topic “The interaction of glycoconjugates and biological status of the organism” (1.08.11.00.03) within the “Differentiated cells in vitro – plant-specific macromolecules, bioprosthesis and models for biological tests (UE 9) (Funding Source: Federal Committee for Science , technology and information; coordinator: INEP, Zemun), led by Prof. dr. Terezija I. Hrženjak (Department of Biology, Faculty of Veterinary Medicine, University of Zagreb)
• From 1989 to 1993. – Associate on the technical project with the IAEA (International Agency for Radiation Protection) and the Ministry of Economy of the Republic of Croatian (CRO / 9/002) “The development of infrastructure for radiation protection and nuclear safety in the Republic of Croatia)
• From 1993-1996. Associate on the topic “The target sites in the human genome associated with the process of carcinogenesis (1-08-329) – this project was included in the program of international cooperation COST 244 Project-” Biomedical effects of electromagnetic radiation “
• From 1998-2002 – Associate on the project “Eco-genetic research activities mutagenic living and working environment with special reference to a specific cell response” (00220107)
• Since 2002 to 2006 Head of the project “Genotoxicity of natural and anthropogenic agents” (0022019) funded by the Ministry of Science, Education and Sport, Republic of Croatia
• From 2005 to 2006 Associate on the technical project “Application of macromolecular formulation (G-90) from earthworm Eisenia foetida tissues in biomedicine” (TP-03 / 009,841), led by Dr. M. Grdiša with RBI; project is financed by the Ministry of Science, Education and Sport, Republic of Croatia
• From 2007 to 2013. Head of the “Genotoxicity of chemical and physical agents of natural and anthropogenic origin” (022-0222148-2137), and under the program 0222148- “Experimental toxicology of natural and synthetic substances”, financed by the Ministry of Science, Education and Sport, Republic of Croatia
• 2011-2013 – Associate on the project: “Monitoring of organic and inorganic pollutants in the environment Plitvice Lakes National Park” (2011-2013) led by Ph.D. Snjezana Herceg Romanic
• 2014-2018 – project associate OPENTOX – HRZZ s (Croatian Science Foundation) – led by Davor Želježić.

Reviewing scientific / technical papers:

• International Journal of Analytical Chemistry
• Arhiv za higijenu rada i toksikologiju
• Invertebrate Survival Journal
• Macedonian Journal of Medical Sciences
• Journal of Applied Toxicology
• Global Science Books – Vermi technology za DSDP
• International Journal of Applied and Basic Medical Research
• Human and Experimental Toxicology